Nutsa Burduli

BSc (Hons) in Biochemistry from University of Galway, Ireland (2011-2015).
Degree project title: Mutation of a key enzyme residue in MSH3 subunit of MutSβ to inactivate its enzymatic function
Principal investigator: Prof. Robert Lahue

MSc in Cancer research from University of Galway, Ireland (2018-2019)
Degree project title: Development of genetically engineered Natural Killer cells for the treatment of Pancreatic Cancer
Principal investigator: Prof. Michael O'Dwyer
Immediate supervisor: Dr. Subhashis Sarkar

PhD in Medical Science from Karolinska Institutet, Stockholm, Sweden (2020-2026)

Thesis title: Unraveling the functional responses of natural killer cells through functional genomics and genetic engineering
Primary investigator: Dr. Evren Alici
Co-supervisors: Assistant Prof. Arnika Kathleen Wagner, Dr. Stephan Meinke, Prof. Hans-Gustaf Ljunggren


Laboratory skills:
Multicolour flow cytometry panel design
Multicolour flow cytometry
Cell culture (primary and cell line)
Cloning
Lentiviral production
Lentiviral transduction
PBMC isolation and culture
PCR
Molecular biology
CRISPR/Cas9-mediated gene knockouts

CRISPR/Cas9-mediated screening
Nucleofection/electroporation
Cytotoxicity assays
Fluorescence assisted cell sorting (FACS) - certified
Western blotting
Gene insert design
DNA/RNA isolation and purification

NK cells
Genetic modification
Lymphocyte proliferation
CRISPR/Cas9 strategies
Lymphocyte biology
Immunotherapies
Cellular immunotherapies

Lentiviral engineering

Promoter engineering

Viral pseudotyping

Previous student: Fernando Herrero Quevedo
Project title: Targeted knockout of amino acid transporters ASCT1 and ASCT2 to abrogate NK cell transduction with baboon envelope pseudotyped lentiviral vectors

Previous student: Kjell Klarenbeek
Project title: Evaluation of candidate genes regulating natural killer cell cytotoxic and cytokine responses