Services
Below you can read about the services offered at the LCI core facility.
Training for researchers
- During our initial discussion, you will receive invaluable advice on experimental and sample design. Together, we will decide which system is best suited for your project.
- You will get a thorough one-to-one training with your own sample. Our aim is that, at the end of training, you have acquired a solid theoretical and practical knowledge of the machine, the software, the sample, the experimental design and the requirements for reliable and reproducible image analysis.
- After you have been trained, you will receive continuous support and guidance, refresher sessions if you have forgotten anything and new expert discussions when your project evolves.
- You will receive professional help to build your image analysis pipeline.
- If your lab has a microscope similar to ours, you are welcome to request a training to increase your expertise, even if you do not plan on using our equipment.
- LCI teaching material and videos
Access to the equipment
- You will have a 24/7 access to the equipment you where trained on. Our training often gives access to several very similar systems, allowing flexibility.
- You will have access to our remote servers with many software for image analysis
- You will have 1 year free access to our OMERO data management system. After 1 yesr, if you want to continue using the OMERO data management system, the usage fee will be 500 kr/year.
Train-the-trainer program
- Staff working at other core facilities are welcome to join our job-shadowing program. They will get the opportunity to follow our user training and discuss pedagogical methods to improve the design of their own end-user training.
Imaging by staff
- If you do not want to be trained but need microscopy images, you are welcome to contact us for the "Imaging by staff" service . You will sit next to us and we will acquire the images you need. The fee for this service is here.
Spatial Transcriptomics
Four Core Facilities in Flemingsberg collaborate and offer 10x Genomics Visium Spatial Transcriptomics on the KI South Campus.
- Feno: cuts and labels the sample
- LCI does the imaging
- SICOF: does the RNA quality control and the transfer to a 10x Genomics slide (regular or HD) then builds the library.
- BEA: sequences at NGI and does the data analysis.
The final price for SICOF and BEA depends on the choice of slide and how much needs to be sequenced. Feno and LCI have standards prices per slide.
Email all 4 core facilities before preparing the sample to get started: singlecellseq@ki.se; phenotype@ki.se; fredrik.fagerstrom-billai@ki.se; LiveCellImaging@ki.se